This study aimed to check the in vitro probiotic properties of eleven Lactobacillus fermentum strains previously isolated from fermented dairy products and infant faeces.
1. IntroductionProbiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host .Lactobacillus spp. are widely used as probiotic bacteria, and their application in foods are generally recognized as safe (GRAS) .Probiotic potential of Lactobacillus spp. is closely related to the cell surface characteristics including cell surface hydrophobicity (CSH
Fermented milk products are a major source of health-promoting microorganisms known as probiotics. To characterize the probiotic properties of lactic acid bacteria isolated from Ghanaian traditionally fermented milk, thirty (30) isolates comprising Enterococcus faecium (1), Lactobacillus fermentum (14), Lb. plantarum (2) and Pediococcus acidilactici (13) identified by 16S rRNA gene sequencing
Altmetric Probiotic Properties of Lactobacillus Species Fermented palm sap is a potential source of LAB. This study aimed to evaluate in vitro antimicrobial and probiotic properties of LAB isolated from traditional fermented palm sap in Thailand. Among 40 isolated LAB species, 10 were preliminarily selected for their antimicrobial activity.
An In Vitro Study of the Probiotic Potential of a Bile This study evaluated the use of a bile-salt-hydrolyzing Lactobacillus fermentum strain as a probiotic with potential hypocholesterolemic properties. The effect of L. fermentum on representative microbial populations and overall metabolic activity of the human intestinal microbiota was investigated using a three-stage continuous culture system.
Oct 21, 2016 · Screening of Probiotic Properties of Lactobacillus Isolates Acid Tolerance. The isolates were incubated overnight in MRS broth at 37°C. Actively grown cells were harvested by centrifugation (7000 rpm, 4°C, 10 min). The pH of MRS broth was adjusted at pH 2.0 with 1N HCl (Ramos et al., 2013). MRS broth adjusted to pH 6.5 was used as a control.
Genome Sequence of Lactobacillus fermentum 47-7, a We report the genome sequence of Lactobacillus fermentum 47-7, a good in vitro probiotic strain isolated from an infant. Its genome size is 1.83 Mb, it is assembled from 180 contigs, and it consists of 1,636 protein-coding genes, 15 rRNAs, 57 tRNAs, and 4 noncoding RNAs. This genome sequence will be useful for a variety of applications.
Identification and in vitro evaluation of probiotic Sep 29, 2020 · The selected cultures DB-1aa, DB-b2-15b, Cu2-PM7, Cu3-PM8 and IB-pM15 were identified by 16S rDNA sequencing as Enterococcus durans, Enterococcus faecium, Lactobacillus plantarum, and two Lactobacillus fermentum, respectively. Several in vitro experiments were carried out including acid and bile tolerance, survival under simulated
Study of the probiotic potential of microorganisms isolated from fermented foods has been increasing, especially studies related to lactobacilli. In intestinal models, lactobacilli have demonstrated beneficial properties, such as anti-inflammatory activity and increased antibody production, but the molecular mechanisms involving probiotic and antagonistic action as well as their effect on
In vitro and in vivo investigations of probiotic Jan 04, 2019 · A total of 88 lactic acid bacteria (LAB) strains were isolated from Chinese traditional sourdough and five of them were selected based on their bile resistance. All the five strains were identified as Lactobacillus plantarum by 16S rRNA gene sequencing. In vitro probiotic properties of the L. plantarum strains including tolerance to simulated gastrointestinal conditions, aggregation
In-vitro characterization of potentially probiotic Nov 08, 2018 · In vitro screening for probiotic properties Acid tolerance. Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products. Food Microbiol 36(1):2229. CAS Article Google Scholar
Probiotic strain of human origin. Lactobacillus fermentum ME-3® was isolated in 1995 at the University of Tartu, Estonia, from a healthy one year old child. The strain displays excellent probiotic properties, resistance to gastro-intestinal environment (bile acids, low pH), lectin profile suitable for adhesion to gut epithelial cells.
Lactobacillus fermentum Probiotics Benefits ProbioticsLactobacillus fermentum RC-14 inhibits Staphylococcus aureus infection of surgical implants in rats. The Journal of infectious diseases. 2002 May 1;185(9):1369-72. Kullisaar T, Songisepp E, Aunapuu M, Kilk K, Arend A, Mikelsaar M, Rehema A, Zilmer M. Complete glutathione system in probiotic Lactobacillus fermentum ME-3.In vitro probiotic properties of Lactobacillus fermentum A lactobacillus strain isolated from a vaginal tract of a healthy woman was examined in vitro for its probiotic potential. This strain, identified as Lactobacillus fermentum SK5, was able to survive at pH 34 and 0.10.2% bile, and unaffected by pepsin (3 g l 1) and pancreatin (1 g l 1), but was susceptible to all tested antibiotics except metronidazole.